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PPTOTO | HowtoUse Red Matter App Flow Cytometry Tutorial

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PPTOTO | HowtoUse Red Matter App Flow Cytometry Tutorial

PPTOTO Getting started with Red Matter App takes only a few steps, and none of them require installing anything. Open the app in your browser and load your FCS file — there's no account to create and no software to download first. As soon as your file is loaded, Red Matter App renders your flow cytometry data as an interactive plot, so you can start exploring your cell populations right away instead of waiting on a desktop program to launch.

From there, the core analysis workflow is the same one researchers use in traditional flow cytometry software, just without leaving your browser. Draw polygon or quadrant gates directly on the plot to isolate the populations you care about, and apply manual compensation to correct for spectral overlap between fluorochromes. Once your gating and compensation are set, you can export your plots for reports, presentations, or publications — taking you from a raw FCS file to a finished figure in just a few clicks.


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FAQ — PPTOTO

Open Red Matter App in your browser and select the option to load a file, then choose the FCS file from your computer. No account or installation is needed — once the file loads, your data appears as an interactive plot ready for analysis.
Once your data is plotted, use the polygon or quadrant gating tool to draw a boundary around the population you want to isolate. Everything inside the gate is treated as that population for further analysis, the same approach used in traditional flow cytometry software.
Red Matter App includes manual compensation tools to correct for spillover between fluorochromes. You adjust the compensation values directly against your plotted data until overlapping signals are properly separated, giving you a cleaner view of each population.
Yes — once you've finished gating and compensating your data, you can export your plots for use in reports, presentations, or publications, so your analysis isn't locked inside the browser session.
First, confirm the file is a valid, uncorrupted FCS file exported directly from your cytometer's software. If it still doesn't load, try refreshing the page or opening Red Matter App in a different modern browser, since browser-based tools can occasionally behave differently across browser versions.

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